• ion channel br Results br Discussion In

  2018-10-29

  

  Results Discussion In our present study we compared the cellular properties of engineered MSCs and naïve MSCs. Several lines of evidence indicate for an absence of significant clonal dominance drift that could potentially have occurred in genetically engineered MSCs during the expansion in vit

• One limitation of using iPSCs is

  2018-10-29

  

  One limitation of using iPSCs is the inability to carry out studies under genetically defined conditions, leading to increased experimental variability. To overcome these obstacles, we used a zinc-finger-nuclease (ZFN)-mediated gene-editing technique (An et al., 2012; Corti et al., 2012; Li et al.,

• dna synthesis Recent reports demonstrate that culture

  2018-10-29

  

  Recent reports demonstrate that culture of mESCs in 2i conditions causes global hypomethylation but spares key regulatory elements, such as imprinted differentially methylated regions (Leitch et al., 2013; Yamaji et al., 2013). Further, gene-expression-array-based hierarchical clustering revealed th

• daunorubicin Most of the observed H K me and H K

  2018-10-26

  

  Most of the observed H3K27me3 and H3K4me3 re-patterning and mRNA expression changes of cardiac genes were associated with the optimal reprogramming condition (MGT, Fig. 3). On the other hand, H3K27me3 deposition at fibroblast genes was observed mostly under the optimal reprogramming condition, while

• br Results br Discussion Many

  2018-10-26

  

  Results Discussion Many small molecules, including two reported by our group (Lee et al., 2013b), have been suggested to be selectively toxic to PSCs. However, as described previously (Knoepfler, 2009), despite the high potency and efficacy of small molecules that target hPSC-specific genes (e

• br Experimental Procedures br Author Contributions All

  2018-10-26

  

  Experimental Procedures Author Contributions All authors fulfill the ICMJE guidelines for authorship. E.B.B., Y.L.T., Y.P.P., S.L., H.S.C., Q.D., and S.-B.J. performed the biological experiments. Y.L.T. and D.R. performed the bioinformatics part of the study. A.L., X.L., C.S., S.-B.J., and U.L

• Several methods have been described to obtain neurons of spe

  2018-10-26

  

  Several methods have been described to obtain neurons of specific subtypes through differentiation of hPSCs, either via formation of three-dimensional (3D) embryoid bodies (EBs) or using monolayers as starting material (Amoroso et al., 2013; Boissart et al., 2013; Boulting et al., 2011; Eiraku and S

• hop over to this site Pacemaking in SAN cells is a complex

  2018-10-26

  

  Pacemaking in SAN hop over to this site is a complex process, explained by a combination of ion fluxes across the sarcolemmal membrane and the SR, designated as the calcium and membrane clocks (Lakatta et al., 2010). The mechanisms of spontaneous beating in hiPSC-CM are not well understood. Previous

• Our study also confirmed that

  2018-10-26

  

  Our study also confirmed that miR-34a overexpression induced the downregulation of NOTCH1 and CYCLIN D1, which have been identified as target genes of miR-34a in tumor cells (Wei et al., 2012; Bae et al., 2012; Li et al., 2012; Sun et al., 2008; Zhang et al., 2014a). In addition, CYCLIN D1 is a dire

• In this study we have developed

  2018-10-26

  

  In this study, we have developed a three-step protocol to differentiate hESCs to endothelial progenitor muscarinic (EPCs) using human recombinant laminins that are biologically relevant (LN521, LN511, and LN421) to mimic the in vivo endothelial substrata. First, vascular cell lineages emerge from m

• br Results Table shows descriptive statistics for

  2018-10-26

  

  Results Table 1 shows descriptive statistics for the full sample. A small percentage (3%) of Malawian children was born premaritally whereas the vast majority of children (69.7%) were born to mothers who married before their birth. There is further diversity in mothers’ marital status: approximat

• cam kinase ii We found little consistency in how adolescent

  2018-10-26

  

  We found little consistency in how adolescent neighbourhood social environments have been both conceptualised and operationalised. When operationalised the various concepts of adolescent neighbourhood measures were largely indistinct. Again, this is similar to findings from the previous review by Sa

• br Background br Methods br

  2018-10-26

  

  Background Methods Results Discussion The hypotheses and whether they were supported are summarized in Table 6. The results indicate that, contrary to prior research on health generally or negative health behaviors such as smoking, own unemployment is not associated with negative changes

• br Acknowledgements This work was supported by NIH

  2018-10-26

  

  Acknowledgements This work was supported by NIH grants 1R01MD006064 and 1R21HD066312 (Dr. Osypuk, PI), and the Minnesota Population Center grant P2C HD041023. Funders did not have any role in design or conduct of the study; collection, management, analysis, and interpretation of the data; or prep

• In healthy individuals during No REM

  2018-10-26

  

  In healthy individuals, during No REM sleep, the metabolic activity decreases on the frontal, temporal and parietal cortices, in contrast with waking levels; individuals with MD do show a non-significant decrease in activity in the same areas of waking during the beginning of sleep. It is possible t

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